Vannina Santoni began her career by interpreting the role of Donna Anna (Mozart's Don Giovanni) in Italy, then at the Royal Opera in Versailles.  After studying at the Conservatoire in Paris, she was quickly noticed for her "getting into her roles with the ferveur of an actress" and her qualities of "pure lyricism mixed with dramatic fire", especially in 2018 when she triumphed in La Traviata in Paris, performing the title role at the Théâtre des Champs Elysées.

In the 2023/24 season, Vannina will make her debut at the Gran Teatre del Liceu as Liu (Turandot). She will then sing Fiordiligi (Cosi fan tutte) at the Zürich Opera and the Opéra de Paris.

The 2022/23 season began with Vannina's debut as Mimi in Puccini's La Bohème at the Théâtre du Capitole in Toulouse. She then reprised the role of Mélisande in Lille under the direction of François-Xavier Roth. She made her debut as Iphigénie in Glück's Iphigénie en Tauride in Montpellier and sang the title role in Massenet's Grisélidis at the Théâtre des Champs-Elysées. At the end of the season, Vannina opened the Rheingau Musical Festival with Poulenc's Stabat Mater, conducted by Alain Altinoglu.

Other highlights from recent seasons include the role of Juliette (Roméo et Juliette, Gounod) at the Teatro alla Scala, Manon with the Orchestre National de Lyon under the baton of Daniele Rustioni and Mélisande at the Théâtre des Champs-Elysées with François-Xavier Roth in the production of Eric Ruf. Also the role of Fiordiligi in Laurent Pelly's new production, with Le Concert d'Astrée conducted by Emmanuelle Haïm at the Théâtre des Champs-Elysées. In 2021 Vannina created the role of Dona Musica in Marc-André Dalbavie’s new opera Le Soulier de Satin at the Paris Opera. She also sang her first Strauss's 4 Last Songs with the Mahlerian Camerata conducted by Benjamin Garzia at the Vichy Opera.